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目的探讨白介素-13(IL-13)、γ-干扰素(IFN-γ)在急性期毛细支气管炎患儿血清中的表达及与病情轻重之间的相关性。方法用酶联免疫吸附(ELISA)法测定2005-02—2005-11于遵义医学院附属医院治疗的42例急性期毛细支气管炎患儿(其中轻症组22例,重症组20例)和16名健康婴儿的血清IL-13、IFN-γ的质量浓度。结果急性期毛细支气管炎患儿血清IL-13的质量浓度[(6.88±2.12)ng/L]明显高于对照组[(5.48±1.28)ng/L,P<0.01]。急性期毛细支气管炎患儿IFN-γ质量浓度[(10.71±2.44)ng/L]明显高于对照组[(9.20±1.54)ng/L,P<0.05];其中轻症组明显高于重症组和对照组(P<0.05),而重症组与对照组差异则无显著性(P>0.05)。结论急性期毛细支气管炎患儿血清IL-13质量浓度明显增高,提示IL-13参与了毛细支气管炎的发病过程,但所测质量浓度不能反映病情严重程度;IFN-γ在轻症毛细支气管炎组质量浓度明显增高,而重症组不增高,这可能与急性重症毛细支气管炎患儿IFN-γ产生受抑制有关。
Objective To investigate the expression of interleukin-13 (IL-13) and interferon-γ (IFN-γ) in sera of children with acute bronchiolitis and their relationship with severity. Methods 42 cases of children with acute bronchiolitis (22 cases in mild group and 20 cases in severe group) and 16 cases in severe group (control group) were enrolled in this study. Enzyme linked immunosorbent assay (ELISA) Healthy infant serum IL-13, IFN-γ mass concentration. Results The serum concentration of IL-13 in children with acute bronchiolitis [(6.88 ± 2.12) ng / L] was significantly higher than that in the control group [(5.48 ± 1.28) ng / L, P <0.01]. The IFN-γ concentration in children with acute bronchiolitis [(10.71 ± 2.44) ng / L] was significantly higher than that in the control group [(9.20 ± 1.54) ng / L, P <0.05] Group and control group (P <0.05), but there was no significant difference between severe group and control group (P> 0.05). Conclusion Serum IL-13 levels in children with acute bronchiolitis were significantly increased, suggesting that IL-13 involved in the pathogenesis of bronchiolitis, but the measured concentration does not reflect the severity of illness; IFN-γ in mild bronchiolitis Group, the concentration was significantly higher, while the severe group did not increase, which may be associated with the occurrence of acute severe bronchitis in children with IFN-γ production was inhibited.