论文部分内容阅读
目的 探讨硒对淋巴细胞抗大肠癌过程中凋亡相关基因Fas/FasL表达的影响。方法 以半胱氨酸硒作为影响因素 ,人T淋巴细胞为效应细胞及人结肠癌细胞 (LoVo细胞株 )为靶细胞 ,采用四甲基偶氮唑蓝 (MTT)比色法、凋亡细胞荧光计数检测凋亡细胞的数量 ,逆转录 聚合酶链 (RT PCR)反应、原位杂交技术检测 ,硒对效应细胞杀伤肿瘤细胞过程中Fas/FasL表达的影响。结果 不同浓度的半胱氨酸硒 (0 .5、1.0mg/L)作用 48h后 ,可增强人T淋巴细胞抗肿瘤活性 [分别为 (2 5 .12± 3 .91) % ,(46 .17± 3 .6 8) % ,P <0 .0 5 ] ,且肿瘤细胞的凋亡比例上升 [分别为(18.6± 4.1) % ,(32 .7± 2 .1) % ,P <0 .0 5 ] ;能使免疫效应细胞和肿瘤细胞表达FasL和Fas水平增高。结论 硒可提高淋巴细胞的抗肿瘤作用 ,可能通过Fas/FasL途径起作用
Objective To investigate the effect of selenium on the expression of apoptosis-related gene Fas/FasL in the process of anti-colorectal cancer. Methods Cysteine selenium was used as an influencing factor. Human T lymphocytes were used as target cells and human colon cancer cells (LoVo cell line) as target cells. MTT colorimetry and apoptotic cells were used. The number of apoptotic cells was detected by fluorescence counting, reverse transcription polymerase chain reaction (RT PCR), in situ hybridization, and the effect of selenium on the expression of Fas/FasL in the process of killing of tumor cells by effector cells. Results The antitumor activity of human T lymphocytes was enhanced by different concentrations of selenium cysteine (0.5, 1.0 mg/L) for 48 h (21.52±3.91 %, respectively) (46. 17 ± 3 . 6 8) %, P <0.05, and the proportion of tumor cell apoptosis increased [(18.6 ± 4.1)%, (32.7% ± 2.1%)%, P <0. 0 5 ] ; It can increase the expression of FasL and Fas in immune effector cells and tumor cells. Conclusion Selenium can increase the anti-tumor effect of lymphocytes and may play a role through the Fas/FasL pathway.