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目的 探讨了槲皮素对人大肠癌细胞SW116生长抑制的影响。方法 采用~3H-TdR掺入法,MTT检测法和双层琼脂集落形成实验,观察了大肠癌细胞的生长变化。结果 槲皮素对人大肠癌细胞SW116有显著的抑制生长及增殖,并能使大肠癌细胞出现脱落并伴有死亡,DNA生成物减少,大肠癌细胞SW116集落形成率降低。结论 本实验研究提示,槲皮素对大肠癌细胞SW116具有较强的抑制生长及杀伤性,并可能运用于协助临床对肿瘤的治疗研究。
Objective To investigate the effect of quercetin on the growth inhibition of human colorectal cancer SW116 cells. Methods The growth of colorectal cancer cells was observed by ~3H-TdR incorporation, MTT assay and double agar colony formation assay. Results Quercetin significantly inhibited the growth and proliferation of human colorectal cancer cell line SW116. It also caused colorectal cancer cells to appear detached and accompanied by death. DNA production decreased, and SW116 colony formation rate decreased. Conclusion The present study suggests that quercetin has a strong inhibitory effect on growth and killing of colorectal cancer cells SW116, and may be used to assist in the clinical treatment of tumors.