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目的探讨硒对淋巴细胞抗结肠癌细胞作用的影响。方法我们以半胱氨酸硒作为影响因素,人T淋巴细胞为效应细胞及人结肠癌细胞(Lovo细胞株)为靶细胞,采用MTT比色法、凋亡细胞荧光计数检测凋亡细胞的数量,并检测上清液中TNFα,SOD和MDA的变化。结果半胱氨酸硒在一定的浓度范围内(0.5mg/L～1.0mg/L),可增强人T淋巴细胞抗肿瘤活性(P=0.042),促进人结肠癌细胞的凋亡,且肿瘤细胞的凋亡比例上升(P=0.006);T淋巴细胞抗肿瘤作用的增强与上清液种中的TNF-α的增高有关(P=0.01),但与SOD和MDA无关(P>0.05)。结论硒可提高淋巴细胞的抗肿瘤作用,且与TNF-α有关。
Objective To investigate the effect of selenium on lymphocyte anti-colon cancer cells. Methods We used cysteine selenium as the influencing factor, human T lymphocytes as effector cells and human colon cancer cells (Lovo cell line) as the target cells. The number of apoptotic cells was detected by MTT assay and apoptotic fluorescent counting , And detected the changes of TNFα, SOD and MDA in the supernatant. Results Selenium in a certain concentration range (0.5mg / L ~ 1.0mg / L), can enhance the anti-tumor activity of human T lymphocytes (P = 0.042), promote human colon cancer cell apoptosis, and the tumor (P = 0.006). The anti-tumor effect of T lymphocytes was related to the increase of TNF-α in supernatant (P = 0.01), but not to SOD and MDA (P> 0.05) . Conclusion Selenium can increase the anti-tumor effect of lymphocytes and is related to TNF-α.