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目的研究木犀草素对正常ICR小鼠脾细胞和S180肉瘤细胞活力的调节作用。方法木犀草素浓度为50、100、200、400μmol/L作用于ICR小鼠脾细胞和S180肉瘤细胞后,采用MTT法检测木犀草素对脾细胞和S180细胞增殖作用的影响;碘化丙啶单染流式检测细胞凋亡情况,流式细胞仪检测细胞内活性氧浓度,羟自由基、DPPH自由基清除实验测定木犀草素清除自由基活性。结果与溶媒对照组比较,200、400μmol/L木犀草素作用ICR小鼠脾细胞后细胞活力增加(P<0.05),100、200、400μmol/L木犀草素作用小鼠S180肉瘤细胞后细胞活力下降(P<0.01);200、400μmol/L木犀草素Sub-G1期脾细胞比例减少(P<0.05),200、400μmol/L木犀草素Sub-G1期S180细胞比例增加(P<0.05)。与溶媒对照组比较,50、100、200及400μmol/L木犀草素作用ICR小鼠脾细胞后内活性氧水平均升高(P<0.05),不同浓度木犀草素作用S180细胞后内活性氧水平均降低(P<0.05)。木犀草素有对羟自由基及DPPH自由基清除活性的作用,且成一定量效关系。结论木犀草素能促进小鼠脾细胞活力,抑制脾细胞凋亡和抑制S180细胞活力,促进S180细胞凋亡的双向调节作用,具有进一步研究开发价值。
Objective To investigate the regulatory effect of luteolin on the viability of splenocytes and S180 sarcoma in normal ICR mice. Methods After the concentration of luteolin was 50,100,200,400μmol / L on ICR mouse spleen cells and S180 sarcoma cells, the effects of luteolin on the proliferation of splenocytes and S180 cells were detected by MTT assay. Propidium iodine Single cell flow cytometry was used to detect apoptosis. Flow cytometry was used to detect intracellular reactive oxygen species (ROS) concentration. Hydroxyl radical and DPPH radical scavenging assay was used to determine the free radical scavenging activity of luteolin. Results Compared with the vehicle control group, the viability of splenocytes of ICR mice treated with 200,400μmol / L luteolin increased (P <0.05), and the cell viability of S180 sarcoma cells treated with 100,200,400μmol / L luteolin (P <0.05). The percentage of S180 cells in 200,400μmol / L sub-G1 phase of luteolin increased (P <0.05) . Compared with vehicle control group, the levels of reactive oxygen species (ROS) in splenocytes of ICR mice treated with 50, 100, 200 and 400 μmol / L luteolin were significantly increased (P <0.05). After treated with different concentrations of luteolin for S180 cells, The levels were lower (P <0.05). Luteolin has a hydroxyl radical and DPPH free radical scavenging activity, and into a certain amount of effect relationship. Conclusion Luteolin could promote the splenocyte vitality, inhibit the apoptosis of splenocytes and inhibit the viability of S180 cells and promote the apoptosis of S180 cells. It is of great value for further research and development.