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目的了解新疆地区不同民族之间丙型肝炎病毒基因分型的分布特征。方法用RT-PCR方法及PCR-荧光探针法对新疆地区278例丙型肝炎患者进行了HCV RNA的检测和基因分型。结果 278例抗HCV阳性标本中,共检出5种基因型。其中1b型170例(61.15%);2a型77例(27.69%);3a型17例(6.12%);3b型13例(4.68%);6a型1例(0.36%)。不同性别中1b型检出率较高,不同性别间基因型2a和3a检出率差异均有统计学意义(P<0.05),不同性别间基因型1b、3b、6a检出率差异无统计学意义(P>0.05)。1b型和2a型在汉族与少数民族之间分布差异有统计学意义(P<0.05)。3a、3b及6a型在汉族与少数民族之间分布差异无统计学意义(P>0.05)。结论新疆地区HCV基因型以1b型为主,基因型分布呈现多样性。
Objective To understand the distribution of hepatitis C virus (HCV) genotypes among different ethnic groups in Xinjiang. Methods HCV RNA was detected and genotyped in 278 hepatitis C patients in Xinjiang by RT-PCR and PCR-fluorescence probe. Results Among 278 anti-HCV positive samples, 5 genotypes were detected. Of which, type 1b was 170 (61.15%), type 2a 77 (27.69%), type 3a 17 (6.12%), type 3b 13 (4.68%) and type 6a 1 (0.36%). The detection rate of genotype 1b and 1b in different genders was higher than that in genotypes 2a and 3a (P <0.05), but there was no significant difference in the genotypes 1b, 3b and 6a among different genders Significance (P> 0.05). The distributions of type 1b and type 2a between Han nationality and ethnic minorities were statistically significant (P <0.05). There was no significant difference in the distribution of types 3a, 3b and 6a between Han nationality and ethnic minorities (P> 0.05). Conclusion The genotypes of HCV genotypes in Xinjiang are mainly genotype 1b, and the genotypes of genotypes are diverse.