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肾宝归真液由淫羊藿、菟丝子、熟地黄等七味中药组成,具有补肾阳,填精益神的作用。该药处方中淫羊藿为主药,淫羊藿苷是淫羊藿的主要有效成分,因此,我们建立了高效液相色谱法测定淫羊藿苷的含量。该法准确,回收率高,重现性好。1 实验方法与结果1.1 仪器与试剂 高效液相色谱仪,美国Waters公司M32系列;紫外可见光分光光度计,2401型日本岛津公司;乙腈色谱纯;淫羊藿苷对照品由中国药品生物制品检定所提供。肾宝归真液（哈药集团三精制药有限公司991221,991222,991223）。
Kidney Po Guizhen Liquid is composed of seven herbs, including Epimedium, Cuscuta, and Rehmannia, and it has the function of tonifying kidney and filling in lean and benefiting God. Epimedin is the main drug in the prescription of this medicine, and icariin is the main active ingredient of epimedium. Therefore, we established a high performance liquid chromatography method for the determination of icariin. The method is accurate, high recovery and good reproducibility. 1 Experimental methods and results 1.1 Instruments and reagents High performance liquid chromatography, Waters Corporation M32 series; UV-Vis spectrophotometer, type 2401 Shimadzu Corporation; acetonitrile chromatographic purity; icariin reference substance from China’s pharmaceutical and biological products test Provided. Shenbao Guizheng Liquid (Harbin Pharmaceutical Group Sanjing Pharmaceutical Co., Ltd. 991221, 991222, 991223).