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目的建立荧光定量逆转录-聚合酶链反应(RT-PCR)方法检测APCDD1 mRNA基因表达的方法,了解直肠癌及结直肠腺瘤组织中APCDD1 mRNA的表达水平。方法用荧光定量PCR方法检测86例直肠癌及癌旁组织、20例结直肠腺瘤及配对的正常黏膜组织APCDD1 mRNA相对表达水平并比较其差异。结果75%的结直肠腺瘤和65%的直肠癌表达上调,86例直肠癌APCDD1 mRNA平均相对表达水平为正常黏膜组的4．712倍,20例结直肠腺瘤中APCDD1 mRNA平均相对表达水平为正常黏膜组的3．872倍,直肠癌组和结直肠腺瘤组均较正常黏膜组织上调,差异有统计学意义(P＜0．05)。结论APCDD1 mRNA表达在腺瘤和直肠癌中表达上调,但在直肠癌中与病理分级无明显相关。
Objective To establish a method for the detection of APCDD1 mRNA expression by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and to understand the expression of APCDD1 mRNA in rectal cancer and colorectal adenoma. Methods The relative expression level of APCDD1 mRNA in 86 cases of rectal cancer, paracancerous tissues, 20 cases of colorectal adenomas and matched normal mucosa tissues was detected by real-time fluorescence quantitative PCR and the differences were compared. Results 75% of colorectal adenomas and 65% of rectal cancers were up-regulated. The average relative expression levels of APCDD1 mRNA in 86 rectal cancer tissues were 4.712 times of those in normal mucosa tissues. The average relative expression levels of APCDD1 mRNA in 20 colorectal adenomas Which was 3.872 times higher than that of the normal mucosa group. Compared with the normal mucosa, the rectal cancer group and the colorectal adenoma group were significantly up-regulated (P <0.05). Conclusion APCDD1 mRNA expression is up-regulated in adenoma and rectal cancer, but not in pathological grade in rectal cancer.