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目的探讨延龄草总皂苷对人结肠癌HT-29细胞凋亡的影响及其机制。方法采用流式细胞术研究延龄草总皂苷对HT-29细胞线粒体膜电位以及凋亡的作用;Western blotting法检测给予HT-29细胞不同浓度延龄草总皂苷后凋亡相关蛋白Caspase-3,8,9,Bax和Bcl-2表达的变化。结果延龄草总皂苷能诱导HT-29细胞的凋亡;延龄草总皂苷可促进HT-29细胞Caspase-3,9及Bax的表达,降低Bc1-2的表达,且均呈剂量依赖性。结论延龄草总皂苷可以通过线粒体途径诱导HT-29细胞凋亡。
Objective To investigate the effect of trillium saponin on apoptosis of human colon cancer HT-29 cells and its mechanism. Methods Flow cytometry was used to study the effect of trillium saponin on mitochondrial membrane potential and apoptosis in HT-29 cells. Western blotting was used to detect the apoptosis-related protein Caspase-3 in HT-29 cells treated with different concentrations of Trichosanthin , 8,9, Bax and Bcl-2 expression. Results Trillium total saponin could induce the apoptosis of HT-29 cells. Trillium saponin could promote the expression of Caspase-3, 9 and Bax and decrease the expression of Bcl-2 in HT-29 cells in a dose-dependent manner . Conclusion Trillium saponin can induce HT-29 cell apoptosis through mitochondrial pathway.