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目的 :以低分化胃腺癌细胞系MGC 80 3为实验对象 ,观察了蟾酥提取物bufalin对其生长、分化的影响。方法 :应用台盼蓝染色法、 76 90 XU荧光染色法和电子显微镜等观察细胞生长、分化。结果 :0 0 1μmol/Lbufalin可显著抑制细胞生长 ,细胞粘附力下降 ,由梭形趋于圆形 ,核质比下降 ,细胞内核糖体和线粒体数目减少 ,内质网不如对照组细胞发达 ;76 90 XU荧光染色显示 ,用药 72h ,约 71%的细胞发生分化。结论 :bufalin能有效诱导胃癌MGC 80 3细胞分化
OBJECTIVE: To investigate the effects of bufalin on the growth and differentiation of poorly differentiated gastric adenocarcinoma cell line MGC 80 3. Methods: Trypan blue staining, 76 90 XU fluorescence staining and electron microscopy were used to observe the cell growth and differentiation. Results: 0 0 1μmol / L bufalin significantly inhibited cell growth and decreased cell adhesion. The spindle shape turned round, the ratio of nuclear to cytoplasm decreased, the number of intracellular ribosomes and mitochondria decreased, and the endoplasmic reticulum was not as developed as the control group. 76 90 XU fluorescent staining showed that about 72% of cells were differentiated after 72 hours. Conclusion: Bufalin can effectively induce gastric cancer MGC 80 3 cells to differentiate