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AIM:To investigate the usefulness of direct hemoperfusion with a polymyxin B-immobilized fiber column (DHP-PMX therapy)for warm hepatic ischemia-reperfusion(I/R)injury after total hepatic vascular exclusion (THVE)using a porcine model. METHODS:Eleven Mexican hairless pigs weighing 22-38 kg were subjected to THVE for 120 min and then observed for 360 min.The animals were divided into two groups randomly:the DHP-PMX group(n=5)underwent DHP-PMX at a flow rate of 80 mL/min for 120 min(beginning 10 min before reperfusion),while the control group did not(n=6).The rate pressure product (RPP):heart rate×end-systolic arterial blood pressure, hepatic tissue blood flow(HTBF),portal vein blood flow (PVBF),and serum aspartate aminotransferase(AST) levels were compared between the two groups. RESULTS:RPP and HTBF were significantly(P< 0.05)higher in the DHP-PMX group than in the control group 240 and 360 min after reperfusion.PVBF in the DHP-PMX group was maintained at about 70%of the flow before ischemia and differed significantly(P <0.05)compared to the control group 360 min after reperfusion.The serum AST increased gradually afterreperfusion in both groups,but the AST was significantly(P<0.05)lower in the DHP-PMX group 360 min after reperfusion. CONCLUSION:DHP-PMX therapy reduced the hepatic warm I/R injury caused by THVE in a porcine model.
AIM: To investigate the usefulness of direct hemoperfusion with a polymyxin B-immobilized fiber column (DHP-PMX therapy) for warm hepatic ischemia-reperfusion (I / R) injury after total hepatic vascular exclusion (THVE) using a porcine model. METHODS: Eleven Mexican hairless pigs weighing 22-38 kg were subjected to THVE for 120 min and then observed for 360 min. The animals were divided into two groups randomly: the DHP-PMX group (n = 5) underwent DHP-PMX at a flow rate of 80 mL / min for 120 min (beginning 10 min before reperfusion) while the control group did not (n = 6) .The rate pressure product (RPP): heart rate × end- systolic arterial blood pressure, hepatic tissue blood flow (HTBF), portal vein blood flow (PVBF), and serum aspartate aminotransferase (AST) levels were more between the two groups. RESULTS: RPP and HTBF were significantly (P <0.05) higher in the DHP-PMX group than in the control group 240 and 360 min after reperfusion. PVBF in the DHP-PMX group was maintained at about 70% of the flow before i (P <0.05) lower than the control group for 360 min after reperfusion. The serum AST was increased afterreperfusion in both groups, but the AST was significantly (P <0.05) lower in the DHP-PMX group CONCLUSION: DHP-PMX therapy reduced the hepatic warm I / R injury caused by THVE in a porcine model.