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目的 探讨IL 2对抗精子抗体 (AsAb)阳性大鼠精子凋亡的影响。方法 建立AsAb阳性大鼠动物模型 ,采用 3′ 原位末端DNA标记 (TUNEL)技术检测大鼠附睾精子凋亡率 ;应用化学比色法测定精子匀浆的总抗氧化能力。结果 AsAb阳性组大鼠精子凋亡率明显高于正常组 (P <0 .0 0 1) ;AsAb +IL 2给药组大鼠精子凋亡率与AsAb阳性组相比显著增高 (P <0 .0 0 1) ;AsAb阳性组大鼠精子匀浆总抗氧化能力与正常组比较显著降低 (P <0 .0 0 1) ;IL 2使AsAb阳性大鼠精子的总抗氧化能力进一步降低 (P <0 .0 0 1)。结论 抗精子抗体阳性大鼠精子凋亡率增加 ;IL 2显著促进精子凋亡 ,可能与氧自由基增多有关
Objective To investigate the effect of IL 2 on sperm apoptosis in spermatozoa antibody-positive (AsAb) -positive rats. Methods The animal model of AsAb positive rat was established. The apoptosis rate of epididymal sperm was detected by 3 ’TUNEL. The total antioxidant capacity of sperm homogenate was determined by chemical colorimetry. Results The sperm apoptosis rate in AsAb positive group was significantly higher than that in normal group (P <0.01). The sperm apoptosis rate in AsAb + IL 2 treated group was significantly higher than that in AsAb positive group (P <0 .0 0 1). The total antioxidant capacity of sperm homogenate in AsAb-positive rats was significantly lower than that in normal rats (P <0.01). IL-2 also decreased the total antioxidant capacity of sperm in AsAb-positive rats P <0 0 0 1). Conclusion Anti-sperm antibody positive rat sperm apoptosis increased; IL 2 significantly promote sperm apoptosis may be related to increased oxygen free radicals