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目的探讨蛋白酶激活受体-2(Protease activated receptor-2,PAR-2)在胃间质瘤组织中的表达及其与胃间质瘤临床病理特征之间的相关性。方法采用RT-PCR、Western blot及免疫组化法检测PAR-2在72例胃间质瘤患者的瘤中心组织、瘤旁组织及正常胃组织中的表达,并分析其与胃间质瘤临床病理特征之间的相关性。结果瘤旁组织和瘤中心组织中PAR-2基因mRNA的转录水平及蛋白的表达水平均明显高于正常组织(P<0.05或P<0.01),瘤中心组织中的表达水平明显高于瘤旁组织(P<0.01)。瘤中心组织中PAR-2基因mRNA的转录水平和蛋白的表达水平均随着NIH分级的升高而上调(P<0.05或P<0.01),且与黏膜受侵情况密切相关(P<0.05)。结论胃间质瘤组织中PAR-2的表达水平明显高于瘤旁组织和正常胃组织,PAR-2的高表达可能与间质瘤的侵袭与转移相关。
Objective To investigate the expression of Protease activated receptor-2 (PAR-2) in gastric stromal tumors and its correlation with the clinicopathological features of gastric stromal tumors. Methods The expression of PAR-2 in 72 cases of gastric stromal tumors was detected by RT-PCR, Western blot and immunohistochemistry in tumor tissue, tumor-adjacent tissues and normal gastric tissues. Correlation between pathological features. Results The mRNA and protein expression of PAR-2 mRNA in tumor-adjacent tissues and tumor-centered tissues were significantly higher than those in normal tissues (P <0.05 or P <0.01). The expression levels of PAR-2 mRNA in tumor tissues were significantly higher than those in tumor tissues Tissue (P <0.01). The transcriptional level and protein expression of PAR-2 mRNA in tumor center tissues were both increased with the increase of NIH grade (P <0.05 or P <0.01), and closely correlated with mucosal invasion (P <0.05) . Conclusions The expression of PAR-2 in gastric stromal tumors is significantly higher than that in adjacent tissues and normal gastric tissues. The high expression of PAR-2 may be related to the invasion and metastasis of stromal tumors.