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[Objective] To compare the chemical components of Anoectochilus spp. from different origins. [Method] The chemical components of three samples of Anoectochilus plants were analyzed and compared by means of LC-MS techniques, which were the tissue cultured A. roxburghii and A. formosanus, and A. roxburghii planted under forest. At the same time, the contents of the main active components in Anoectochilus plants were compared and analyzed by anthrone-sulfuric acid method and ultraviolet spectrophotometry, including polysaccharides and total flavonoids. [Result] Polysaccharides contents in three samples of Anoectochilus (HNJ201001, HNJ201002 and HNJ201003) were 16.175%, 19.636% and 10.939%; while the total flavonoids contents were 0.722%, 0.895% and 0.984%, respectively. [Conclusion] The results showed that the chemical components and their contents of these three samples were quite similar. Of which, the contents of total flavonoids of the three samples were similar to or even higher than those of the wild species. As for the tissue cultured A. roxburghii and A. formosanus, polysaccharides contents were far higher than the wild species.
[Objective] To compare the chemical components of Anoectochilus spp. From different origins. [Method] The chemical components of three samples of Anoectochilus plants were analyzed and compared by means of LC-MS techniques, which were the tissue cultured A. roxburghii and A At the same time, the contents of the main active components in Anoectochilus plants were compared and analyzed by anthrone-sulfuric acid method and ultraviolet spectrophotometry, including polysaccharides and total flavonoids. [Result] Polysaccharides contents in three samples of Anoectochilus (HNJ201001, HNJ201002 and HNJ201003) were 16.175%, 19.636% and 10.939%; while the total flavonoids contents were 0.722%, 0.895% and 0.984%, respectively. [Conclusion] The results showed that the chemical components Of which, the contents of total flavonoids of the three samples were similar to or even higher than those of the wild species. As for the tissue cultured A. roxburghii and A. formosanus, polysaccharides contents were far higher than the wild species.