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药代动力学研究中迫切需要一种实时在位检测方法。本文提出一种新的基于荧光光谱技术的实时在位检测方法,并搭建了一套用于大鼠体内药代动力学研究的监测系统,得到荧光强度和染料浓度的关系曲线,对大鼠体内荧光染料Cypate(近红外多次甲基菁染料)进行实时检测,并和体外荧光成像系统的结果比较来验证分析系统的可行性。结果表明:(1)Cypate浓度在0.098～25μg/ml范围内线性回归方程为y=73.249x+130.97(R2=0.999 1,P<0.001),高、中、低浓度的RSD分别为1.23%、6.29%和13.48%,检测灵敏度达到0.098μg/ml,特异性好;(2)Cypate的浓度变化与体外成像系统的结果基本一致(r=0.992 5),很好地反映了它在大鼠体内的代谢过程。论文的结论是为药代动力学研究提供了一种新的实时在位检测方法。
Pharmacokinetic studies urgently need a real-time in-position detection method. In this paper, we present a new real-time in-position detection method based on fluorescence spectroscopy and set up a monitoring system for the pharmacokinetics of rats in vivo. The fluorescence intensity and dye concentration curves were obtained. Fluorescence The dye Cypate (NIR Polymerase) was tested in real time and compared with the in vitro fluorescence imaging system to verify the feasibility of the analytical system. The results showed that: (1) The linear regression equation of Cypate concentration in the range of 0.098-25μg / ml was y = 73.249x + 130.97 (R2 = 0.999 1, P <0.001). The RSDs of high, medium and low concentrations were 1.23% 6.29% and 13.48%, respectively. The detection sensitivity was 0.098μg / ml, and the specificity was good. (2) The change of Cypate concentration was in good agreement with the in vitro imaging system (r = 0.992 5) Metabolic process. The conclusion of the dissertation provides a new real-time in-position detection method for pharmacokinetic study.