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目的:优化生化丸的提取工艺并建立薄层鉴别和高效液相测定有效成分的最佳方法,为定量和定性分析生化丸的质量提供有效手段和可靠依据。方法:采用TLC法对生化丸中主要药材当归、川芎、甘草进行鉴别;采用HPLC法测定生化丸中阿魏酸的含量,色谱柱:Agilent 5 TC-C_(18)(2)(250 mm×4.6 mm,5μm);流动相:甲醇-5.0%冰醋酸溶液(25∶75);检测波长:322 nm;流速:1.0 ml·min~(-1);柱温:室温。结果:TLC图谱中可检出当归、川芎、甘草的特征图谱,阿魏酸质量浓度在1.28~20.51μg·ml~(-1)内线性关系良好,r=0.999 9;平均回收率为98.47%,RSD=0.36%(n=6)。结论:采用此种方法,能高效提取生化丸中有效成分,方法可行、准确、重复性好,可用于生化丸的质量控制。
OBJECTIVE: To optimize the biochemical pill extraction process and establish the best method for TLC identification and HPLC determination of active ingredients, which can provide an effective means and a reliable basis for quantitative and qualitative analysis of the quality of Biochemical Pills. Methods: The contents of ferulic acid in Biochemical Pills were determined by TLC. The main components of Angelica, Chuanxiong and Glycyrrhiza were identified by TLC. The chromatographic column was Agilent 5 TC-C 18 (2) (250 mm × 4.6 mm, 5 μm); mobile phase: methanol-5.0% glacial acetic acid (25:75); detection wavelength: 322 nm; flow rate: 1.0 ml · min -1; Results: The chromatograms of Angelicae Sinensis, Rhizoma Chuanxiong and Glycyrrhizae could be detected in TLC. The linear range of the concentration of ferulic acid was 1.28 ~ 20.51μg · ml ~ (-1), r = 0.999 9; the average recovery was 98.47% , RSD = 0.36% (n = 6). Conclusion: By this method, the effective components in the biochemical pills can be efficiently extracted. The method is feasible, accurate and reproducible and can be used for the quality control of the biochemical pills.