论文部分内容阅读
我们从1980年起开展了化学诱变剂(EMS)处理春小麦种子、合子和配子的试验研究,比较不同处理的诱变效果,以期找出其中合适而有效的处理方法,同时对处理后代的变异情况进行了观察和分析。材料与方法供试品种和品系为“新曙光1号”、77—1017”、“鉴7”,均为遗传性稳定的原种材料。化学诱变剂 EMS 的浓度为0.3%,用磷酸盐缓冲液(pH 7)为溶剂配制而成。处理配子和尚未分裂的合子细胞均在田间进行。具体方法:在处理前选择生长发育良好而健壮的具有品种典型性的植株,将其中部即将开裂的麦穗,剪去芒和颖尖,除去穗上部、下部的小穗,留中部8～10个小穗,同时将所留小穗中间的小花摘除,可直接进行配子处理;如用于合子处理,则整穗后还要去雄和人工授粉,以使合子细胞的发育
Since 1980, we conducted a pilot study on the treatment of spring wheat seeds, zygotes and gametes by chemical mutagens (EMS), and compared the mutagenic effects of different treatments in order to find suitable and effective treatment methods, and at the same time deal with the variation of offspring The situation was observed and analyzed. MATERIALS AND METHODS The varieties and lines tested were “New Dawn 1”, 77-1017 “and” Jian 7 ", all of which were hereditary and stable original materials.The concentration of chemical mutagen EMS was 0.3% The buffer solution (pH 7) was formulated as a solvent, and the gametes and unorganized zygote cells were treated in the field.Methods: Select plants with good growth and robustness typical of the species before treatment, Of the ears, cut off Mans and Ying tip, remove the upper part of spikelets, the lower part of the spikelets, leaving the middle of 8 to 10 spikelets, while leaving spikelets in the middle of the flowers removed, can be directly gametes; such as for Zygote Treatment, but also after the whole ear to male and pollination, so that the development of zygote cells