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目的 :观察并比较缬沙坦及苯那普利对 5 6肾切除大鼠肾小球硬化的改善作用 ,探讨其作用机制。方法 :选用SD雄性大鼠 30只 ,通过 5 6肾切除法制造慢性肾功能衰竭模型 ,术后 2周随机分为模型组、缬沙坦组及苯那普利组 ,并设假手术组作为对照。术后第 6周末各组大鼠进行体重、血压、血清肌酐 (Scr)及尿素氮 (BUN)的测定 ,处死大鼠 ,取出肾组织进行病理组织形态学观察 ,并采用免疫组织化学方法检测肾组织转化生长因子 β1(TGF β1)、IV型胶原、纤维连接蛋白 (FN)表达。结果 :同模型组相比 ,两治疗组收缩压下降 (P均 <0 .0 1) ,肾功能改善 ;肾小球系膜增殖程度、肾小球硬化指数 (GSI)降低 (P均 <0 .0 1) ;免疫组化染色显示 ,两治疗组肾小球转化生长因子 β1(TGF β1)、纤维连接蛋白 (FN)及IV型胶原表达均较模型组下降 (P均 <0 .0 1)。结论 :缬沙坦及苯那普利对慢性肾功能衰竭大鼠具有同等的肾脏保护作用 ;改善肾小球硬化是通过减少肾小球TGF β1表达 ,从而减少FN及IV型胶原生成来实现的。
Objective: To observe and compare the effects of valsartan and benazepril on glomerulosclerosis in 56 nephrectomized rats and its mechanism. Methods: Thirty SD male rats were used to make chronic renal failure model by 56 nephrectomy. The rats were randomly divided into model group, valsartan group and benazepril group after 2 weeks. Control. At the end of the sixth week after operation, the body weight, blood pressure, serum creatinine (Scr) and blood urea nitrogen (BUN) of rats in each group were measured. The rats were sacrificed and the renal tissues were removed for histopathological observation. Immunohistochemistry Tissue transforming growth factor β1 (TGF β1), type IV collagen, fibronectin (FN) expression. Results: Compared with the model group, systolic blood pressure (P <0.01), renal function improved, glomerular mesangial proliferation and glomerular sclerosis index (GSI) decreased .0 1). Immunohistochemical staining showed that the expressions of TGFβ1, FN and type IV collagen in the two treatment groups were significantly lower than those in the untreated group (P <0.01) ). CONCLUSIONS: Valsartan and benazepril have the same renal protective effect on rats with chronic renal failure. Improving glomerular sclerosis can be achieved by reducing the expression of TGF-β1 in glomeruli, thereby reducing the formation of FN and type IV collagen .