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作者介绍用免疫磁技术从人骨髓中分离提纯巨核细胞的新方法。从健康胸骨或髂后上嵴吸取10ml骨髓,用酸性枸缘酸葡萄糖(ACD-A)和EDTA抗凝继加同体积的MK 培养液混合,调正至pH7.3、渗透压290MOSM/L。骨髓细胞悬液用两种不同密度的percoll 液分层,密度分别为1.020g/ml 和
The authors introduce a new method for the isolation and purification of megakaryocytes from human bone marrow using immunomagnetic techniques. From the healthy sternum or posterior superior iliac crest 10ml of bone marrow aspirated with acid citrate dextrose (ACD-A) and EDTA anticoagulant followed by mixing the same volume of MK culture medium was adjusted to pH7.3, osmolality 290MOSM / L. Bone marrow cell suspensions were layered with two different concentrations of percoll fluid at densities of 1.020 g / ml and