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用D一半乳糖诱发了大鼠白内障,分析了其膜固有蛋白和尿素溶蛋白的改变。对正常大鼠晶状体纤维细胞膜蛋白电泳图谱的分析表明,其26KDa的主要膜固有蛋白(MIP26)约占膜固有蛋白总量的42.9％,22KDa的膜固有蛋白约占6.4％。糖性白内障大鼠晶状体纤维细胞膜固有蛋白以22KDa为主,约占30.5％,MIP26几乎消失。并在24KDa处有一深染区带。在白内障发病过程中18KDa的膜蛋白的相对含量无明显改变。这些结果提示在糖性白内障发病过程中MIP26发生了降解,产生了22KDa的降介产物。 以每克湿重计,白内障晶状体尿素溶蛋白降低约22.5％。但白内障晶状体尿素溶蛋白占总蛋白的比例升高。正常大鼠尿素溶蛋白电泳图谱主要显示四条区带,其近似分子量分别为22.5KDa、22.4KDa、20.6KDa和19.2KDa。白内障晶状体20.6KDa和19.2KDa的二条区带所占的比例明显减小。这个结果提示正常晶状体尿素溶蛋白主要含有α—和β—晶体蛋白,而且白内障晶状体尿素溶蛋白则主要含有β—晶体蛋白。
Rat cataract was induced with D-galactose and the changes of its membrane-bound proteins and urea-soluble proteins were analyzed. Analysis of normal rat lens fibromatin membrane protein electrophoresis showed that its 26KDa major membrane-associated protein (MIP26) accounted for about 42.9% of the total membrane-bound proteins, and 22KDa membrane-associated proteins accounted for about 6.4%. The intrinsic protein of lens fiber cell membrane of 22KDa was the main protein in 30% of diabetic cataract rats, MIP26 almost disappeared. And a deep stained zone at 24 kDa. In the pathogenesis of cataract 18KDa membrane protein relative content of no significant change. These results suggest that MIP26 is degraded during the development of glyco-cataract and produces a 22 kDa down-regulated product. Cataract lenin decreased about 22.5% on a gram wet weight basis. However, cataract lens urea protein in the proportion of total protein increased. Normal rat urinary protein proteolytic pattern mainly shows four bands with approximate molecular weights of 22.5KDa, 22.4KDa, 20.6KDa and 19.2KDa, respectively. The proportion of the two bands of 20.6KDa and 19.2KDa in cataract lens significantly decreased. This result suggests that normal crystalline urea predominantly contains alpha- and beta-crystallin, and that cataract crystallin predominantly contains beta-crystallin.