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目的:以休眠芽为外植体,建立麻花秦艽丛生芽再生的实验技术体系,为麻花秦艽种苗工厂化离体快繁和种质资源离体保存提供理论依据和技术支撑。方法:以MS为基本培养基,添加不同浓度和种类的细胞分裂素和生长素,诱导休眠芽产生丛生芽。以1/2MS为基本培养基,添加不同浓度和种类的生长素诱导丛生芽生根。结果:MS+2.0 mg/L 6-BA+0.01 mg/L NAA+30 g/L蔗糖+7 g/L琼脂培养基是诱导休眠芽产生丛生芽及丛生芽继代的最佳培养基,丛生芽诱导率为93.3%,丛芽经继代培养后芽增殖系数为5.6。最佳生根培养基是1/2MS(无机盐减半)+2.0 mg/L IAA+0.5 mg/L IBA+15 g/L蔗糖+7 g/L琼脂,生根率达93.5%,再生植株生长健壮。结论:该试验建立了以休眠芽为外植体的麻花秦艽丛生芽再生植株的技术体系。
OBJECTIVE: To establish an experimental system for the regeneration of shoots of Gentiana macrophylla with dormant buds as explants, which provides a theoretical basis and technical support for in vitro rapid propagation and germplasm conservation of Gentiana straminea plants. Methods: With MS as the basic medium, cytokinin and auxin with different concentrations and types were added to induce dormant buds to produce cluster buds. With 1 / 2MS as the basic medium, different concentration and kinds of auxin were added to induce rooting of cluster buds. Results: MS + 2.0 mg / L 6-BA + 0.01 mg / L NAA + 30 g / L sucrose + 7 g / L agar was the best media for inducing dormancy buds to produce the next generation of cluster buds and cluster buds. The bud induction rate was 93.3%. The bud multiplication coefficient of the bud bud after subculture was 5.6. The best rooting medium was 1 / 2MS (inorganic salt half) +2.0 mg / L IAA + 0.5 mg / L IBA + 15 g / L sucrose +7 g / L agar with the rooting rate of 93.5% . Conclusion: This experiment established a dormant bud as explants of the genus Tamarix spinosa regenerated plant technology system.