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Objectives To investigate the effect of telmisartan on human umbilical vein endothelial cells(HUVEC) exposed to high glucose in vitro and the related mechanism.Methods HUVECs were incubated with telmisartan and glucose(5 mmol/L,30 mmol/L) at 0 h,12 h,24 h,36 h,48 h,respectively.The level of malondialdehyde(MDA) and superoxide dismutase(SOD) in the supernatant of cultured endothelial cells was measured by thiobarbituric acid test and xanthine oxidase test.The expression of PPAR-γwas determined at 24 hour with Western blot technique.Results When the endothelial cells were cultured in high glucose environment,the MDA level was significantly increased,but the SOD activity and the protein expression of PPAR-γwere markedly decreased.However,the high glucose-induced effects were inhibited by telmisartan intervention.Conclusion Telmisartan can decrease oxidative stress and increase PPAR-γexpression of endothelial cells in high glucose environment.
Objectives To investigate the effect of telmisartan on human umbilical vein endothelial cells (HUVEC) exposed to high glucose in vitro and the related mechanism. Methods HUVECs were incubated with telmisartan and glucose (5 mmol / L, 30 mmol / L) at 0 h, The levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the supernatant of cultured endothelial cells was measured by thiobarbituric acid test and xanthine oxidase test. The expression of PPAR- γwas determined at 24 hour with Western blot technique. Results when the endothelial cells were cultured in high glucose environment, the MDA level was significantly increased, but the SOD activity and the protein expression of PPAR-γwere markedly decreased. However, the high glucose- induced effects were inhibited by telmisartan intervention. Conclusions Telmisartan can decrease oxidative stress and increase PPAR-γ expression of endothelial cells in high glucose environment.