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小麦愈伤组织的诱导频率在小麦花药组培中有着极其重要的作用。本文探讨了三种温度处理（低温处理、包括预处理和后处理；高温后处理；低温预处理和高温后处理两者的配合处理）对春小麦愈伤组织的诱导频率后发现；多数品系的接种穗在４℃冰箱内进行三～六天的低温预处理，能够提高出愈率，预处理超过六天，大部分花粉都已过了最佳接种时期，使出愈率下降。低温预处理的诱导效果明显地要比低温后处理的好。接种后的花药经过六～九天的高温后处理，出愈率可比对照提高二──四倍。并且综合两年的结果比较了低温预处理，高温后处理，以及低温预处理和高温后处理两者配合的出愈率，发现高温后处理的诱导效果最好，低温预处理的效果相对较差，两者配合作用的效果处于两个单独处理之间。最后讨论了花药经短期较高温度培养对白苗率的影响。
The induction frequency of wheat callus plays an extremely important role in wheat anther culture. In this paper, the induction frequency of three kinds of temperature treatments (low temperature treatment, including pretreatment and post treatment, high temperature post treatment, low temperature pretreatment and high temperature post treatment) on the callus induction of spring wheat was investigated. Most strains were inoculated Spike in the refrigerator at 4 ℃ for three to six days of low temperature pretreatment, can increase the healing rate, pretreatment more than six days, most of the pollen have been the best time of inoculation, so that the rate of decline. The induction effect of low temperature pretreatment is obviously better than the low temperature post treatment. Anther after inoculation after six to nine days of high temperature post-treatment, the cure rate can be increased two times than the control ─ ─ four times. And the results of two years compared the low temperature pretreatment, high temperature post-treatment, as well as low temperature pretreatment and high temperature post-treatment with the combination of the two therapies, found that high temperature post-treatment induced the best, low-temperature pretreatment effect is relatively poor , With the effect of the two in between the two separate treatment. Finally, the effect of anther culture on the rate of white sprouts by short-term higher temperature was discussed.