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目的 探讨甲状腺癌组织中 p5 3基因点突变。 方法 应用非同位素PCR -SSCP技术检测 39例不同类型甲状腺癌石蜡包埋组织中 p5 3基因点突变。 结果 10例低分化甲状腺癌中 4例 (4 0 % )、9例未分化甲状腺癌中 6例 (67% )出现异常电泳 ,表明这些病例相应DNA片段中发生了点突变 ,而 5例乳头状癌和 5例滤泡型癌均未见该基因的突变。 10例出现异常电泳的甲状腺癌中 9例 (90 % )同时呈现 p5 3蛋白的表达。 结论 非同位素PCR -SSCP是一简便、快速、有效的检测基因点突变的方法 ,p5 3基因点突变率与蛋白表达率基本相一致 ,p5 3基因的突变是分化型癌向间变型癌转化的关键性遗传改变。
Objective To investigate the mutation of p5 3 gene in thyroid cancer. Methods Non-isotopic PCR-SSCP was used to detect the mutation of p5 3 gene in 39 cases of different types of thyroid carcinoma. Results Of the 10 cases with poorly differentiated thyroid carcinoma, 4 cases (40%) and 9 cases of undifferentiated thyroid carcinoma (6 cases) (67%) showed abnormal electrophoresis, indicating that the corresponding DNA fragments in these cases had a point mutation, while 5 cases of papillary No mutations in this gene were found in all cancers and in 5 follicular carcinomas. Nine cases (90%) of 10 thyroid carcinomas with abnormal electrophoresis also showed the expression of p5 3 protein. Conclusion Non-isotope PCR-SSCP is a simple, rapid and effective method to detect point mutations. The point mutation rate of p5 3 gene is basically consistent with the protein expression rate. The mutation of p5 3 gene is the transition from differentiated carcinoma to metachronous carcinoma Key genetic changes.