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目的检测非酒精性脂肪肝患者血清瘦素与血小板磷脂脂肪酸,分析其相关性。方法先用薄层层析法分离血小板磷脂,将分离出的磷脂甲酯化后提取甲酯化脂肪酸,气相色谱分析各种甲酯化脂肪酸的含量,采用放免法检测NAFLD组和对照组的血清瘦素。结果 NAFLD组的血清瘦素(3.52±1.31)ng/ml显著高于对照组(2.92±0.98)ng/ml,总n-6(30.86±2.97)%高于对照组(29.02±3.67)%,而血小板磷脂总n-3(7.07±2.25)%低于对照(8.24±2.72)%;血清瘦素与血小板磷脂总n-3负相关(r=-0.312,P<0.01),与总n-6正相关(r=0.332,P<0.01),控制年龄、性别、HOMA、BMI等因素后,相关仍有意义。结论饮食脂肪酸对非酒精性脂肪肝患者血清瘦素水平有调节作用。
Objective To detect serum leptin and platelet phospholipid fatty acids in patients with non-alcoholic fatty liver and analyze its correlation. Methods The platelet phospholipids were separated by TLC, the methyl esterified fatty acids were extracted by methyl esterification and the contents of various methyl esterified fatty acids were analyzed by gas chromatography. The serum levels of NAFLD and control were detected by radioimmunoassay Leptin. Results Serum leptin (3.52 ± 1.31) ng / ml in NAFLD group was significantly higher than that in control group (2.92 ± 0.98 ng / ml, 30.86 ± 2.97% vs 29.02 ± 3.67% While the total n-3 (7.07 ± 2.25)% of platelet phospholipids was lower than that of control (8.24 ± 2.72)%. Serum leptin was negatively correlated with total n-3 of platelet phospholipids (r = -0.312, 6 (r = 0.332, P <0.01). After controlling for age, gender, HOMA, BMI and other factors, the correlation is still significant. Conclusion Dietary fatty acids have a regulatory effect on serum leptin levels in patients with nonalcoholic fatty liver disease.