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目的了解宁波地区福氏c亚型志贺菌病原学特征、耐药性和毒力基因分布,为细菌性痢疾的有效防控提供依据。方法收集来自腹泻患者的志贺菌株,对其进行生化鉴定、血清分型、药敏试验和5种毒力基因检测。结果 149株菌株中福氏(B群)志贺菌占63.76%,其中福氏1c、2c和4c亚型占36.91%;宋内(D群)志贺菌占36.24%;未检出A群和C群志贺菌。福氏1c、2c和4c亚型菌株生化结果与ATCC标准菌株一致;血清凝集抗原结构式分别为Ⅰ∶7(8)、Ⅱ∶3,4∶7(8)和Ⅳ∶7(8),均为多耐药性菌株;5种毒力基因(ipa H、set1、sen、ial和vir A)的阳性率分别为100.00%、85.45%、85.45%、90.91%和87.27%。43株菌株(78.18%)携带全部毒力基因。结论福氏c亚型志贺菌已成为宁波地区主要流行株之一,除血清变异外,生化等生物学特性未变异,菌株为多重耐药菌,携带多种毒力基因,有较强的致病力,应加强志贺菌检测与菌型分析。
Objective To understand the etiological characteristics, drug resistance and virulence genes of Shigella flexneri in Ningbo and to provide basis for effective prevention and control of bacillary dysentery. Methods Shiga strains from patients with diarrhea were collected and biochemically identified, serotyped, drug sensitive and 5 virulence genes tested. Results The 149 strains of Shigella flexneri (group B) accounted for 63.76%, of which 36.91% were Freund’s 1c, 2c and 4c subtypes, and 36.24% were in Song dynasty (group D). A group And C group Shigella. The biochemical results of the two strains were consistent with the standard strains of ATCC. The structural formulas of serum agglutinated antigen were Ⅰ: 7 (8), Ⅱ: 3, 4: 7 (8) and Ⅳ: 7 Were multi-resistant strains. The positive rates of five virulence genes (ipa H, set1, sen, ial and vir A) were 100.00%, 85.45%, 85.45%, 90.91% and 87.27%, respectively. 43 strains (78.18%) carry all the virulence genes. Conclusion Shigella flexneri C subtype Shigella has become one of the major epidemic strains in Ningbo. In addition to the variation of serum, the biochemical and other biological characteristics are not different. The strain is multi-drug resistant and carries a variety of virulence genes with strong Virulence should be strengthened Shigella detection and analysis of bacteria.