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目的研制庆大霉素-藻酸钙三维缓释微球并调控其庆大霉素的释放,使其达到长期局部抗菌的效果。方法制作不同浓度T、S、U组庆大霉素-藻酸钙缓释凝珠,与庆大霉素-骨水泥颗粒Y组进行庆大霉素释放情况比较。通过不同时间点抽取浸泡液,送紫外分光光度法(UV)及金黄色葡萄球菌(SA)培养检测,由此计算出各组包封率、释放率,绘制庆大霉素释放曲线。结果 4组样品(微生物法)的包封率及30 d药物释放率分别为:U组(53.99%、36.31%),S组(39.62%、27.55%),T组(34.20%、30.83%),Y组(100.00%、48.49%)。U组的包封率较高,30 d释放庆大霉素的总量较大,更接近Y组。4组间差异有统计学意义(P<0.01)。U、Y组庆大霉素的释放明显高于S、T组的释放。各组30 d内庆大霉素的浓度几乎都能超过金黄色葡萄球菌的最低抑菌浓度(MIC),即>2μg/ml。结论 U组载药缓释凝珠30 d内的庆大霉素释放较为理想,可作为BMSCs的三维培养支架。
Objective To develop gentamicin - calcium alginate three - dimensional sustained - release microspheres and control the release of gentamicin to achieve long - term local antibacterial effect. Methods Gentamicin-calcium alginate sustained-release beads with different concentrations of T, S and U were prepared and compared with gentamicin-bone cement granules Y group for gentamicin release. The soaking liquid was extracted at different time points, and then UV and UV-visible spectrophotometry (UVA) and Staphylococcus aureus (SA) culture were used to detect the encapsulation efficiency and release rate of each group. Gentamicin release curve was drawn. Results The entrapment efficiency and drug release rate of the four groups of samples (microbiological method) in the four groups were respectively 53.99%, 36.31% in group U, 39.62%, 27.55% in group S, 34.20%, 30.83% in group T, , Y group (100.00%, 48.49%). The entrapment efficiency of group U was higher, and the total amount of gentamicin released at 30 days was larger and closer to group Y. The difference between the 4 groups was statistically significant (P <0.01). The release of gentamicin in group U and Y was significantly higher than that in group S and T. The concentration of gentamicin within 30 days in each group almost exceeded the minimum inhibitory concentration (MIC) of Staphylococcus aureus, ie> 2μg / ml. Conclusion The release of gentamicin in group U sustained-release gels within 30 d is ideal and can be used as a three-dimensional scaffold for BMSCs.