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目的 探讨非小细胞肺癌 (NSCLC)中细胞周期蛋白D1(CyclinD1)的表达及其临床意义。方法 用鼠抗人CyclinD1单克隆抗体(DCS/6 )对 89例石蜡包埋的原发性NSCLC组织进行免疫组化染色 ,并与 2 0例肺炎性病变比较。结果 CyclinD1在肺鳞癌、腺癌、大细胞癌中都有较高的表达 ,其阳性率分别为 6 0 .5 %、6 1.0 %、40 .0 % ;而在肺炎性病变中 ,则全部阴性。在肺鳞癌、腺癌中 ,肿块直径≥ 3cm、有淋巴结转移或远处转移者 ,其CyclinD1阳性率较高 (P <0 .0 5 ) ,且与临床分期有关 ,Ⅲ～Ⅳ期癌中的阳性率高于Ⅰ～Ⅱ期癌 (P <0 .0 5 )。结论 CyclinD1的过表达与NSCLC的发生、发展和预后等密切相关 ,对NSCLC的诊断、治疗有一定的指导意义 ,可作为一个诊断及预后指标。
Objective To investigate the expression of cyclin D1 in non-small cell lung cancer (NSCLC) and its clinical significance. Methods 89 cases of paraffin-embedded primary NSCLC tissues were immunohistochemically stained with mouse anti-human CyclinD1 monoclonal antibody (DCS / 6) and compared with 20 cases of pneumonia lesions. Results CyclinD1 was highly expressed in lung squamous cell carcinoma, adenocarcinoma and large cell carcinoma with positive rates of 60.5%, 6 1.0% and 40.0%, respectively. However, in the case of pneumonia, all negative. In lung squamous cell carcinoma and adenocarcinoma, the positive rate of CyclinD1 was higher in patients with lymph node metastasis or distant metastasis (P <0.05), and was related to clinical stage. In patients with stage Ⅲ-Ⅳ carcinoma The positive rate was higher than Ⅰ ~ Ⅱ stage cancer (P <0.05). Conclusion The overexpression of CyclinD1 is closely related to the occurrence, development and prognosis of NSCLC, which may be helpful for the diagnosis and treatment of NSCLC. It may serve as a diagnostic and prognostic indicator.