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A new near-infrared fluorescent dye, 9-N-(2-hydroxyethyl)-N-methylamino-6-carbethoxy- 5 H-benzo[a] phenoxazin-5-one 1, was prepared from the reaction of N - (2 - hydroxyethyl)-N -methyl -4 -nitrosoaniline hydrochloride and ethyl 1, 3 - dihydroxynaphthoate. Five more fluorescent compounds were synthesized by the reaction of the resulting dye 1 with appropriate amino acid or carboxylic acids.