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目的：了解疟原虫的多胺代谢与咯萘啶（ＰＮＤ）抗药性的关系。方法：感染伯氏疟原虫ＡＮＫＡ株（ＰＳ）和由该株培育的中抗ＰＮＤ品系（ＰＲＡ）及高抗ＰＮＤ品系（ＰＲＢ）的昆明株小鼠于腹腔接种（ｉｐ）后ｄ７取血，经薄层层析后用荧光分光光度法测定正常ＲＢＣ、ＰＳ、ＰＲＡ和ＰＲＢ感染ＲＢＣ的丁二胺（ＰＴＣ）、精脒（ＳＰＤ）和精胺（ＳＰＭ）量。另有感染ＰＳ和ＰＲＢ的小鼠于ｉｐ后ｄ６分别１次灌胃（ｉｇ）ＰＮＤ５ｍｇ／ｋｇ和１０ｍｇ／ｋｇ，ｄ７取血，按上述方法测定给药后感染ＲＢＣ的多胺量，并与不给药组比较。结果：ＰＳ感染ＲＢＣ的多胺量均明显高于未感染疟原虫的正常ＲＢＣ，而感染ＰＲＡ和ＰＲＢ的ＲＢＣ多胺量又显著高于ＰＳ感染ＲＢＣ，且多胺量的增高与抗性程度有关。经ＰＮＤ治疗后ＰＳ感染ＲＢＣ的ＳＰＤ和ＳＰＭ较未治疗组显著下降，而ＰＲＢ感染ＲＢＣ则未见明显变化。结论：伯氏疟原虫对ＰＮＤ的抗药性与其多胺代谢有关。
Objective: To understand the relationship between polyamine metabolism of Plasmodium and resistance to pyronaridine (PND). Methods: The Kunming strain of mice infected with Plasmodium berghei ANKA (PS), the medium resistant PND strain (PRA) and the high resistant PND strain (PRB) were inoculated intraperitoneally (ip) Thin layer chromatography was used to determine the amount of butanediamine (PTC), spermidine (SPD) and spermine (SPM) in RBCs infected with normal RBC, PS, PRA and PRB by fluorescence spectrophotometry. In addition, mice infected with PS and PRB were dosed intragastrically (ig) with PND 5 mg / kg and 10 mg / kg once a day d ip, respectively, and d7 blood was collected. The amount of polyamine in RBC after administration was determined as above, Administration group comparison. Results: The amount of polyamine in RBC infected with PS was significantly higher than that in normal RBC without P. falciparum infection, while the amount of polyamine in RBC infected with PRA and PRB was significantly higher than that in PS-infected RBC, and the increase of polyamine was related to the degree of resistance . Compared with untreated group, the SPD and SPM of PS-infected RBC decreased significantly after PND treatment, but there was no obvious change of PRB-infected RBC. Conclusion: The resistance of Plasmodium berghei to PND is related to polyamine metabolism.