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Objective Probe into the influence and the mechanisms of CCK and gastrin on the apoptosis of bile duct carcinoma cells. Methods By taking beauvericin as the revulsant to the apoptosis of bile duct carcinoma cells, the influence of CCK and gastrin on the apoptosis of bile duct carcinoma cells was investigated by using the techniques such as TUNEL fluorescent staining, stream mode cell detecting instrument and reverse bcl-2 oligonucleotide. Techniques of immunohistochemistry, in situ hybridization, flow cytometry (FCM) , RT-PCR were used to study the roles of apoptosis-related genes bcl-2 and baxResults After beauvericin 40 uM worked for 12 h, the survival rate of QBC939 bile duct carcinoma cells was decreased by 35% ?40% . About 80% of the bile duct carcinoma cells showed various degrees of apoptosis. CCK and gastrin could upregulate the threshold value of the apoptosis of bile duct carcinoma cells, which could be inhibited by L60, L18 and reverse bcl-2 oligonucleotide. In terms of both transcription and
Objective Probe into the influence and the mechanisms of CCK and gastrin on the apoptosis of bile duct carcinoma cells. Methods By taking beauvericin as the revulsant to the apoptosis of bile duct carcinoma cells, the influence of CCK and gastrin on the apoptosis of bile duct carcinoma cells were investigated by using the techniques such as TUNEL fluorescent staining, stream mode cell detecting instrument and reverse bcl-2 oligonucleotide. Techniques of immunohistochemistry, in situ hybridization, flow cytometry (FCM), RT-PCR were used to study the roles of apoptosis 40%. About 80% of the bile duct carcinoma cells showed various degrees of apoptosis. CCK and gastrin could upregulate the threshold value of the apoptosis of bile duct carcinoma cells, which could be inhibited by L60, L18 and reverse bcl-2 oligonucleotide. transcription and