论文部分内容阅读
Objective: To elucidate the functional characterization of miR-218 in hepatocellualar carcinoma. Methods: miR-218 mimics and anti-miR-218 were transfected into HCC cells, and the cell proliferation and cell cycle were analyzed by MTT assays and flow cytometry. Lv-miR-218 were constructed and miR-218 overexpression stable HCC cells were generated. The colony formation was assayed and the in vivo tumorigenesis was examined using xenograft tumor model in nude mouse. Results: miR-218 overexpression inhibited cell proliferation and induced cell cycle arrest in vitro, and in vivo study showed that miR-218 suppressed tumorigenesis in nude mouse. Conclusions: miR-218 may be a promising molecular target for HCC therapy.