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目的:探讨携P-选择素抗体靶向脂质微泡评价睾丸完全缺血/再灌注损伤的可行性。方法:制备普通脂质微泡(MB)和携P-选择素抗体靶向微泡(MBp)。30只大白兔随机分成对照组、缺血/再灌注损伤(IRI)0.5 h组、1 h组、2 h组、4 h组,每组6只,在缺血/再灌注术后行MB和MBp的双侧睾丸造影(间隔20 min),经过4～5 min待健侧睾丸实质中MB或MBp完全消失,测量术侧睾丸第一帧超声造影的声强度(F-P,10-5AU)。随后将术侧睾丸取下行免疫组化分析。结果:MBp造影图像显示各IRI组术侧睾丸F-P值分别大于各组的MB(P均<0.05),并在2 h[MBp(8.34±1.20)10-5AU,MB(1.87±0.25)10-5AU]最高。对照组术侧睾丸MB、MBp声强度未见明显差异(均为0 AU)(P>0.05)。免疫组化示对照组睾丸血管内皮无明显P-选择素表达,而IRI组P-选择素表达增加,并随时间延长而增多。结论:携P-选择素抗体靶向微泡超声造影能够评价睾丸IRI的炎症反应。
Objective: To investigate the feasibility of using P-selectin antibody targeting lipid microvesicles to evaluate the complete ischemia / reperfusion injury in testis. Methods: Preparation of common lipid microbubbles (MB) and P-selectin antibody targeting microbubbles (MBp). Thirty rabbits were randomly divided into control group, ischemia / reperfusion injury (IRI) 0.5 h group, 1 h group, 2 h group, 4 h group, with 6 rats in each group. After ischemia / reperfusion, The intensity of the first testis ultrasound (FP, 10-5 AU) was measured by bilateral testicular angiography (interval 20 min). The MB or MBp disappeared completely in 4 ~ 5 min after 4 ~ 5 min. Then the side of the testis removed immunohistochemical analysis. Results: The MBp contrast images showed that the FP values of the testis in each IRI group were significantly higher than those in each group (P <0.05), and were significantly higher at 2 h [MBp (8.34 ± 1.20) 10-5 AU, MB (1.87 ± 0.25) 5AU] the highest. There was no significant difference in the testis MB MB and MBp sound intensity in the control group (all 0 AU) (P> 0.05). Immunohistochemistry showed that there was no significant P-selectin expression in the testicular vascular endothelium in the control group, while P-selectin expression increased in the IRI group and increased with time. CONCLUSION: Contrast-enhanced microbubble contrast-enhanced immunosorbent assay with P-selectin antibody can evaluate the IRI inflammatory response in testis.