近三年来,我所科技人员和职工,土法上马,土洋结合,大胆革新,进行烤烟单倍体试验研究.在简化培养基、改进培养方法和染色体加倍等方面取得了可喜的成果.先后共进行了830管,4,300个花药培养,其中形成胚状体1,376个,诱导率32%,分化小苗660株,分化频率达48.2%.有五个品系花粉植株,通过染色体加倍已结实,加倍率一般5～8%,高者达58%.其中以(Fue347×401)杂种一代用花粉培育筛选育成的“云花一号”,经过作为 In the past three years, scientific and technical personnel and workers of our institute have been encouraged by local laws and regulations, and have made bold innovations in conducting experiments on haplogeny of flue-cured tobacco. We have made gratifying achievements in simplifying culture medium, improving cultivation methods and doubling chromosomes A total of 830 tubes and 4,300 anther cultures were obtained, of which 1,376 embryoid bodies were formed, the induction rate was 32% and the differentiation seedlings were 660. The frequency of differentiation reached 48.2% .Five strains of pollen plants were confirmed to have doubled chromosome doubling rate 5 ~ 8% in general and 58% in height, among which, the hybrid F1 generation (Fue347 × 401) was pollinated with “Yunhua-1”