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目的检测分泌型卷曲相关蛋白2(SFRP2)基因在急性髓系白血病(AML)患者中的甲基化状态,探讨SFRP2基因启动子区甲基化状态与AML发生的关系。方法收集了99例AML患者的骨髓或外周血样本,匹配以70例门诊普通患者的外周血作为对照。采用甲基化特异性PCR(MSP)方法对所有研究样本进行SFRP2基因启动子区甲基化状况检测。结果 99例AML患者中有27例检测到SFRP2基因的甲基化,甲基化率为27.3%;而正常对照组中未检出SFRP2基因的甲基化;两者比较差异有统计学意义(P<0.001)。SFRP2基因的甲基化状态与AML的年龄、性别、临床分型及样本来源(骨髓/外周血)间均未见显著关系(P>0.05)。结论 SFRP2基因启动子区的甲基化与AML的发生有相关性,可能是引起AML的分子机制之一。外周血中的SFRP2基因的甲基化可望作为一项新的分子标记物应用于AML的临床早期检测。
Objective To detect the methylation status of secretory Frizzled Related Protein 2 (SFRP2) gene in patients with acute myeloid leukemia (AML) and to explore the relationship between the methylation status of SFRP2 gene promoter region and the occurrence of AML. Methods The bone marrow or peripheral blood samples from 99 patients with AML were collected and matched with the peripheral blood of 70 outpatients in general as control. Methylation-specific PCR (MSP) was used to detect the promoter methylation status of SFRP2 gene in all samples. Results The methylation of SFRP2 gene was detected in 27 out of 99 cases of AML patients with a methylation rate of 27.3%. However, no methylation of SFRP2 gene was detected in the normal control group. The difference was statistically significant ( P <0.001). There was no significant relationship between the methylation status of SFRP2 gene and the age, sex, clinical type and source of samples (bone marrow / peripheral blood) of AML (P> 0.05). Conclusion The methylation of SFRP2 gene promoter region is associated with the occurrence of AML, which may be one of the molecular mechanisms that cause AML. The methylation of SFRP2 gene in peripheral blood is expected to be used as a new molecular marker in clinical early detection of AML.