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1982～1985年共接种兰科兰属中国兰品种45个,诱导成功20个品种(其中素心兰品种11个)。接种外植体(茎尖、侧芽)917个,诱导启动率为35.3％,诱导成功率为13.6％。研究表明,从9～13cm长的新芽上切取的外植体的启动、成功率最高;对供试材料的特殊管理,可有效地降低外植体的污染率;诱导、增殖过程中的褐化死亡是影响诱导成功率的关键,采用防褐变的综合措施,有明显效果;不同品种和培养阶段对培养基、培养条件的要求不尽相同。通过四年的研究,初步掌握‘雪兰’、‘朱砂’等20个中国兰品种快速繁殖建立无性繁殖系的技术。
A total of 45 Chinese orchid cultivars from Lancaster were inoculated from 1982 to 1985, and 20 varieties were successfully induced (including 11 Supran). Inoculation explants (shoot tips, lateral buds) 917, the induction rate was 35.3%, the successful induction rate was 13.6%. The results showed that the explants cut from 9-13 cm long shoots had the highest success rate of initiation and success. The special management of the tested materials could effectively reduce the contamination rate of explants; browning during induction and propagation Death is the key factor that affects the success rate of induction. The comprehensive measures of anti-browning have obvious effect. The requirements of culture medium and culture conditions are different in different varieties and culture stages. Through four years of research, we have initially mastered the technology of rapid propagation of 20 Chinese blue cultivars such as ’Shenlan’ and ’Cinnabar’ to establish asexual propagation line.