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目的分析电压门控性氯通道ClC家族中的ClC-2氯通道在大鼠小梁网中的表达,探讨ClC-2在青光眼发病机制中的可能作用。方法经前房内注射玻璃酸钠建立大鼠慢性高眼压模型,取正常大鼠及模型大鼠小梁网组织,采用RT-PCR法检测ClC-2基因mRNA的转录水平,免疫组化法检测ClC-2蛋白的表达水平。结果在正常大鼠和慢性高眼压模型大鼠小梁网组织中,均可检测到ClC-2的表达,且模型大鼠ClC-2基因mRNA的转录水平和蛋白的表达水平均较正常大鼠降低。结论大鼠小梁组织中存在ClC-2氯通道的表达,该通道的表达受小梁网的一些病理因素的影响。
Objective To analyze the expression of ClC-2 chloride channel in the ClC family of voltage-gated chloride channels in rat trabecular meshwork and to explore the possible role of ClC-2 in the pathogenesis of glaucoma. Methods Chronic intraocular hypertension (IOP) model was established by intramuscular injection of sodium hyaluronate into the anterior chamber. The normal and rat models of trabecular meshwork were taken. The transcription level of ClC-2 mRNA was detected by RT-PCR. Immunohistochemistry The expression of ClC-2 protein was detected. Results The expression of ClC-2 was detected in the trabecular meshwork of normal rats and chronic ocular hypertension model rats, and the mRNA and protein expression of ClC-2 mRNA in the model rats were higher than normal Lower rat. Conclusion The expression of ClC-2 chloride channel exists in the trabecular tissue of rats. The expression of this channel is influenced by some pathological factors of the trabecular meshwork.