荧光偏振（ｆｌｕｏｒｅｓｃｅｎｃｅｐｏｌａｒｉｚａｔｉｏｎ）的实验研究始于１９２９年Ｐｅｒｒｉｎ［１］对荧光小分子在溶液中寿命的测定．直到二十年后，这一技术才被Ｗｅｂｅｒ等［２］用于对蛋白质分子运动的研究．自此以后，荧光偏振技术在分子生物学特别是蛋白... Fluorescence polarization (fluorescencepolarization) experimental study began in 1929 Perrin [1] for the determination of fluorescent small molecules in solution life. It was not until 20 years later that this technique was used by Weber et al. [2] to study the movement of protein molecules. Since then, fluorescence polarization technology in molecular biology especially protein ...