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目的 用巢式 PCR法检测马拉色菌特异的 DNA从而快速准确的检测并鉴定马拉色菌的各菌种 ,并应用于花斑癣 (Pityriasis Versicolor,PV)患者皮屑中马拉色菌携带情况中的研究。方法 从 PV患者的皮屑中提取马拉色菌的 DNA,以马拉色菌属 r RNA内转录间隔区设计属的引物和各个种的特异性引物分别进行巢式多聚酶链反应 (nest-PCR) ,检测 10 5例 PV患者皮屑中的马拉色菌的目的 DNA片断。结果 10 5例 PV患者共检测到 5例种的共 32 1株马拉色菌的 DNA。每例患者平均检测到约 3种马拉色菌的 DNA。各种马拉色菌的检出率依次为 :球形马拉色菌 80 .0 % ,限制马拉色菌 81.9% ,合轴马拉色菌 77.1% ,糠秕马拉色菌 4 1.9% ,钝形马拉色菌 15 .2 %。未检测到厚皮马拉色菌和斯洛菲马拉色菌。结论 PV皮屑中存在多种马拉色菌 ,球形马拉色菌、合轴马拉色菌和限制马拉色菌是 PV患者皮损处的主要马拉色菌种
Objective To detect Malassezia specific DNA by nested PCR and to detect and identify Malassezia strains rapidly and accurately, and to apply Malassezia flavescens to the dander of Pityriasis Versicolor (PV) Carrying case studies. Methods The DNA of Malassezia was extracted from dander of PV patients. The genomic DNA of Malassezia r RNA was designed and the specific primers of each species were used for nested polymerase chain reaction ), Detection of 105 cases of PV patients with dander dander in the DNA fragment of interest. Results Totally 32 DNAs of Malassezia were detected in 5 out of 105 PV patients. An average of about 3 DNAs of Malassezia were detected per patient. The detection rates of various Malassezia strains were as follows: 80.0% of Malassezia globosa, 81.9% of Malassezia, 77.1% of Malassezia coxarum, 1.9% of Malassezia furfur 4, Malassezia 15.2%. Malassezia furfur and P. sophori were not detected. Conclusion There are many kinds of Malassezia globosum in PV dander, and Malassezia globosa, Malassezia coxis and Malassezia majus are the main Malassezia strains in the lesions of PV patients