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澳大利亚南澳发展研究所(SARDI)针对大田小麦土传病害,研究开发出一套农作物主要土传病原物高通量定量监测与风险预警系统。这一系统包括通过室内外试验,建立病原菌数量与危害程度风险评估分级标准;土样采集;自每份土样(500 g)中提取总DNA;设计异性引物和探针;实时定量PCR(Q-PCR)分析总DNA中各靶标病原物DNA数量。按照风险评估标准,分别做出各种病原物对下茬作物可能的危害程度,在播种前提出相应的防治措施和建议。该系统已在南澳、威尔士等主要小麦生产区广泛应用,对小麦9种土传病原物进行定量监测和预警的有偿技术服务。
Australia’s South Australian Institute of Development (SARDI) has developed a set of high-throughput quantitative monitoring and risk early warning systems for the major soil-borne pathogens of crops against the soil-borne diseases of the field. This system includes the establishment of grading standards for the risk assessment of the number and hazard of pathogens through indoor and outdoor tests; the collection of soil samples; the extraction of total DNA from 500 g of soil samples; the design of heterosexual primers and probes; -PCR) to analyze the total DNA in each target pathogen DNA quantity. According to the standard of risk assessment, the potential hazards of various pathogenic agents to the stubble crops are separately made, and corresponding prevention measures and suggestions are put forward before sowing. The system has been widely used in major wheat producing areas such as South Australia and Wales to provide paid technical services for quantitative monitoring and early warning of 9 soil-borne pathogens of wheat.