应用过量胸腺嘧啶方法,对人体肝癌细胞系Hep 3B的早中期核型进行G显带分析。研究表明(Hep3B)细胞系的染色体众数为57～73,每个细胞中有正常染色体55～64条,异常染色体9～15条,其中可确认其结构的标记染色体13条(包括缺失、易位和等臂染色体等),95%细胞中X染色体为2条,Y染色体为1条。上述现象对认识肝癌细胞系的染色体畸变提供了细胞遗传学某些实验依据。 G-banding analysis was performed on the early and mid-term karyotypes of human hepatocellular carcinoma cell line Hep 3B using the excess thymidine method. Studies have shown that (Hep3B) cell line chromosome number of 57 to 73, each cell has a normal chromosome 55 to 64, abnormal chromosome 9 to 15, which can confirm the structure of the marker chromosome 13 (including the deletion, easy And other arm chromosomes, etc.), 95% of the cells in the X chromosome 2, Y chromosome 1. The above phenomenon provides some experimental evidence for cytogenetics in understanding the chromosomal aberrations of liver cancer cell lines.