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考虑到蛋白质在结晶过程中溶液和晶体之间的动态平衡 ,蛋白质在溶液中的聚合状态决定了晶体堆积的最小单元 .通过动态光散射实验以及计算机模拟 ,对大鼠金属硫蛋白亚型 和兔肝金属硫蛋白亚型 和 的聚合物形式进行了比较 ,可以确定大鼠金属硫蛋白亚型 在结晶条件下的缓冲液中应该以四聚体而不是二聚体形式存在 .通过考察晶体的模型也可以发现 ,晶体的确可以通过四聚体的形式堆积得到 ,用四聚体堆积模型可以对晶体堆积作出更为合理的解释
Considering the dynamic equilibrium between solution and crystal in the process of crystallization, the polymerization state of the protein in solution determines the smallest unit of crystal packing.Dynamic light scattering experiments and computer simulations are performed on the rat metallothionein subtype and rabbit Comparison of the hepatic metallothionein subtype with the polymer form confirms that the rat metallothionein subtype should exist as a tetramer rather than as a dimer in the buffer under crystallization conditions.Through investigating the crystal’s model It can also be found that the crystals can indeed be deposited in the form of tetramers, and the tetramer accumulation model can give a more reasonable explanation of the crystal packing