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目的:研究中药清肝饮对人成纤维细胞(HLF)、大鼠肝贮脂细胞(Ito细胞)增殖活性的影响。方法:采 用胶原酶灌流,密度梯度离心方法分离大鼠肝Ito细胞,以3H-胸腺嘧啶(3H-TdR)和3H-脯氨酸(3H-Pro)的 掺入法观察细胞增殖效应。结果:清肝饮对HLF、Ito细胞具有显著的抑制细胞增殖和胶原合成作用,并随清肝 饮浓度的升高而作用增强。结论:清肝饮对肝纤维化相关细胞HLF、Ito细胞增殖活性具有抑制作用。
Objective: To study the effect of Qingganyin on the proliferation of human fibroblasts (HLF) and rat hepatic fat-storing cells (Ito cells). METHODS: Rat hepatic Ito cells were isolated by perfusion with collagenase and density gradient centrifugation. Cell proliferation was observed by 3H-thymidine (3H-TdR) and 3H-Proline (3H-Pro) incorporation. RESULTS: Qinggan Decoction had significant effects on the inhibition of cell proliferation and collagen synthesis in HLF and Ito cells, and the effect was enhanced with the increasing concentration of Qinggan Decoction. Conclusion: Qinggan Decoction has an inhibitory effect on the proliferation of liver fibrosis-related cells HLF and Ito cells.