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目的探讨microRNA-34a在肺癌细胞凋亡中的作用。方法将A549肺癌细胞株分为3组:microRNA-34a转染组、对照组和未转染组。分析microRNA-34a在A549肺癌细胞中的表达,microRNA-34a对A549肺癌细胞生长周期、细胞增殖、细胞凋亡的影响,microRNA-34a对A549肺癌细胞Bcl-2、P53和C-MYC蛋白以及mRNA表达的影响。结果 miRNA-34a转染组microRNA-34a的表达明显高于对照组(P<0.05)。miRNA-34a转染组G0/G1细胞明显高于对照组和未转染组(P<0.05)。miRNA-34a转染组48 h和96 h的增殖率明显低于对照组和未转染组(P<0.05)。miRNA-34a转染组的细胞凋亡明显高于对照组和未转染组(P<0.05)。miRNA-34a转染组的Bcl-2蛋白和C-MYC蛋白表达水平明显低于对照组(P<0.05);miRNA-34a转染组的P53蛋白表达水平明显高于对照组(P<0.05)。miRNA-34a转染组的Bcl-2 mRNA和C-MYC mRNA表达水平明显低于对照组Bcl-2 mRNA和C-MYC mRNA表达水平(P<0.05);miRNA-34a转染组的P53 mRNA表达水平明显高于对照组P53 mRNA表达水平(P<0.05)。结论 microRNA-34a抑制A549肺癌细胞增殖,促进A549肺癌细胞的凋亡,机制可能为下调Bcl-2和C-MYC基因,上调P53基因。
Objective To investigate the role of microRNA-34a in lung cancer cell apoptosis. Methods A549 lung cancer cell lines were divided into 3 groups: microRNA-34a transfected group, control group and untransfected group. The effect of microRNA-34a on A549 lung cancer cells growth cycle, cell proliferation and apoptosis was analyzed. The expression of Bcl-2, P53 and C-MYC proteins and mRNA The impact of expression. Results The expression of miRNA-34a in miRNA-34a group was significantly higher than that in control group (P <0.05). G0 / G1 cells in the miRNA-34a transfected group were significantly higher than those in the control and untransfected groups (P <0.05). The proliferation rates of miRNA-34a transfected cells at 48 h and 96 h were significantly lower than those in the control and untransfected groups (P <0.05). The apoptosis of the miRNA-34a transfected group was significantly higher than that of the control group and the untransfected group (P <0.05). The expression of Bcl-2 protein and C-MYC protein in miRNA-34a transfected group was significantly lower than that in control group (P <0.05). The expression of P53 protein in miRNA-34a transfected group was significantly higher than that in control group (P <0.05) . The expression of Bcl-2 mRNA and C-MYC mRNA in the miRNA-34a transfected group was significantly lower than that in the control group (P <0.05). The expression of P53 mRNA in the miRNA-34a transfected group The level of P53 mRNA in the control group was significantly higher than that in the control group (P <0.05). Conclusion MicroRNA-34a can inhibit the proliferation of A549 lung cancer cells and promote the apoptosis of A549 lung cancer cells. The possible mechanism is downregulation of Bcl-2 and C-MYC genes and upregulation of P53 gene.