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目的 :在致妊高征 (PIH)众多因素中 ,观察PIH孕妇外周血及其脐血姐妹染色单体互换 (SCE)频率和内皮素(ET)的变化及其两者的关系 ,以资探讨PIH的病因和提高PIH的监测水平。方法 :采用SCE法和放免法分别对 2 1例PIH病人和 15例正常孕妇 (对照组 )的外周血及其所分娩新生儿脐血SCE频率及其ET进行测定。结果 :PIH组外周血和脐血SCE频率分别为 6 .11± 1.2 9,5 .98± 1.38;对照组外周血和脐血SCE分别为 3 .45± 0 .71,3.16± 0 .5 7,其两组比较 ,差异有极显著性 (P均 <0 .0 0 1)。PIH组外周血和脐血SCE频率皆异常。PIH组外周血ET含量的均值 ( 5 1.87± 2 4.72pg/ml)明显低于其脐血的均值 ( 6 3 .6 5± 2 7.6 3pg/ml) ,两者比较差异有显著性 (P <0 .0 5 )。两组SCE和ET相关性分析无明显相关性 (r=0 .0 92 ,P >0 .0 5 )。结论 :PIH与遗传物质DNA损伤有关 ,SCE频率可作为一项诊断、预测PIH的重要指标。PIH孕妇脐血ET增高有助于PIH发病机理的揭示
OBJECTIVE: To observe the changes of sperm chromatin (SCE) frequency, endothelin (ET) and peripheral blood of pregnant women with PIH in pregnant women with PIH and the relationship between the two, To explore the etiology of PIH and improve the monitoring of PIH. Methods: SCE and radioimmunoassay were used to determine the frequency of SCE and ET in peripheral blood of 21 PIH patients and 15 normal pregnant women (control group), respectively. Results: The frequency of SCE in peripheral blood and umbilical cord blood of PIH group were 6.11 ± 1.2 9,5 .98 ± 1.38 respectively; the SCE of peripheral blood and umbilical cord blood of control group were respectively 3.45 ± 0.71, 3.16 ± 0.57 The differences between the two groups were significant (P <0.01). PIH group peripheral blood and cord blood SCE frequency are abnormal. The mean ET level of peripheral blood in PIH group (5 1.87 ± 2.72 pg / ml) was significantly lower than that of umbilical cord blood (63.56 ± 2 7.6 3 pg / ml), the difference was significant (P < 0 .0 5). There was no significant correlation between SCE and ET in the two groups (r = 0.92, P> 0.05). Conclusion: PIH is related to DNA damage of genetic material. The frequency of SCE can be used as an important index for diagnosis and prediction of PIH. PIH pregnant women with elevated uterine blood can help reveal the pathogenesis of PIH