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研究了极低氧(0.2、1.0、2.0 mg/L)、低氧(3.0、5.0 mg/L)和高氧(11.0、14.0 mg/L)处理28 d期间,大菱鲆幼鱼外周血红细胞核异常以及血清超氧化物岐化酶(SOD)、过氧化氢酶(CAT)活力和肝脏丙二醛(MDA)含量等氧化抗氧化平衡中参数的变化。结果显示,极低氧条件下,0.2、1.0和2.0 mg/L组的实验鱼分别在处理2 h、1 d和2 d内全部死亡,其余各组则没有出现死亡现象。溶解氧(DO)对大菱鲆核异常包括微核及总核异常率的影响表现出明显的时间和剂量累积效应,到28 d时才在5.0、14.0 mg/L组和对照组中(正常充气,约7.0 mg/L)观察到微核现象。恢复正常充氧14 d后,高氧组和对照组中仍有微核现象出现。2 h时极低氧组SOD活力较高。其他各处理时间时,SOD活力在各组之间差异显著。各组CAT活力在2 h时未发生明显变化,在7、28和42 d时差异显著(P<0.05)。极低氧组大菱鲆肝脏MDA含量较低,低氧组和高氧组MDA含量均随着处理时间的延长逐渐降低。恢复后低氧组MDA含量显著升高(P<0.05)。研究还发现,对照组大菱鲆也会受到过氧化的胁迫作用,而5.0 mg/L组的总核异常率和所测氧化还原指标在整个实验期间均未发生明显变化。
The effects of very low oxygen (0.2,1.0,2.0 mg / L), low oxygen (3.0, 5.0 mg / L) and high oxygen (11.0,14.0 mg / L) Abnormalities and the parameters in the balance of oxidation and anti-oxidation such as serum superoxide dismutase (SOD), catalase (CAT) activity and hepatic malondialdehyde (MDA) content. The results showed that under the condition of very low oxygen, the experimental fish of 0.2, 1.0 and 2.0 mg / L groups all died within 2 h, 1 d and 2 d of treatment, and no death occurred in the other groups. Dissolved oxygen (DO) showed a significant time and dose cumulative effect on nuclear anomalies including micronucleus and total nuclear anomalous rate in turbot, and it was not significant at 28 days in the 5.0, 14.0 mg / L and control groups Inflated, about 7.0 mg / L) micronuclei were observed. After 14 days of normal oxygenation, there were still some micronuclei in the hyperoxia group and the control group. In the hypoxia group, SOD activity was higher at 2 h. At other treatment times, SOD activity varied significantly among the groups. The CAT activity in each group did not change significantly at 2 h, and the difference was significant at 7, 28 and 42 d (P <0.05). In the hypoxia group, the content of MDA in the liver of turbot turbot was lower than that in the hypoxia group. The content of MDA in the hypoxia group and the hyperoxia group decreased gradually with the prolongation of treatment time. The content of MDA in hypoxia group was significantly increased after recovery (P <0.05). The study also found that turbot in the control group was also subjected to the stress of peroxidation, while the total nuclear anomalous rate and the measured redox index in 5.0 mg / L group did not change significantly during the whole experiment.