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用膜片钳技术研究了Transwell小室趋化迁移后的鼻咽癌CNE-2Z细胞容积激活性CT电流。47％低渗刺激迁移后的CNE-2Z细胞诱发容积激活性氯电流,与未迁移细胞相比,其特性以及其对氯通道阻断剂的敏感性发生明显的变化,此电流的密度明显高于未迁移细胞,而且该电流几乎完全被氯通道阻断剂adenosine-5’-triphosphate(ATP,10 mmol/L)、5-nitro-2-3-phenylpropylamino benzoic acid(NPPB,100μmol/L)和他莫昔芬(30μmol/L)抑制,其中NPPB和他莫昔芬对迁移细胞的抑制作用明显强于未迁移细胞。迁移后的CNE-2Z细胞容积激活性氯通道对阴离子的通透性为:Br>Cl>I>葡萄糖酸,与未迁移细胞(I>Br>Cl>葡萄糖酸)不同。结果提示,容积激活性氯通道可能参与CNE-2Z细胞的迁移过程。
Patch-clamp technique was used to study the cell volume-activated CT current of nasopharyngeal carcinoma CNE-2Z after transwell chemotaxis. The volume-activated chloride current induced by 47% hypotonic stimulation of CNE-2Z cells induced a significant change in its characteristics and its sensitivity to chloride channel blockers compared to non-migrated cells, with a markedly increased density of this current The cells were not migrated and the currents were almost completely blocked by the chloride channel blockers adenosine-5’-triphosphate (ATP, 10 mmol / L), 5-nitro-2-3- phenylpropylamino benzoic acid Tamoxifen (30μmol / L) inhibition, in which NPPB and tamoxifen on the migration of cells was significantly stronger than the non-migrated cells. The permeability of CNE-2Z cell volume-activated chloride channel to anion was: Br> Cl> I> gluconic acid, which was different from non-migrated cells (I> Br> Cl> gluconic acid). The results suggest that volume-activated chloride channels may participate in the migration of CNE-2Z cells.