目的:探讨AZFc/DAZ基因缺失与不育患者精子生成障碍的关系。方法:应用多重PCR技术对无精子症217例和严重少精子症89例患者的外周血细胞进行DAZ基因缺失检测。结果:共发现32例有DAZ基因缺失,其中无精子症21例,缺失率为9.68%;严重少精子症11例,缺失率为12.36%。sY84,sY86及ZFX/Y扩增均为阳性。60例正常生育男性均无DAZ缺失。结论:DAZ基因是AZF重要的候选基因,DAZ微缺失可能是引起无精子和严重少精子并造成男性不育的最重要原因之一。 Objective: To investigate the relationship between AZFc / DAZ gene deletion and infertility in patients with spermatogenesis. Methods: DAZ gene deletion was detected in peripheral blood cells from 217 cases of azoospermia and 89 cases of severe oligospermia using multiplex PCR. Results: There were 32 cases of DAZ gene deletion, including 21 cases without azoospermia, the deletion rate was 9.68%; 11 cases of severe oligospermia, the deletion rate was 12.36%. sY84, sY86 and ZFX / Y amplification were positive. There were no DAZ deletions in 60 normal fertile men. Conclusion: The DAZ gene is an important candidate gene of AZF. Microdeletion of DAZ may be one of the most important causes of azoospermia and severe oligozoospermia and cause male infertility.