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目的:研究大鼠给予大黄及调胃承气汤后,大黄酸在大鼠体内药动学过程。方法:大鼠分别给予大黄及调胃承气汤,高效液相色谱法测定大黄酸的血药浓度。色谱柱为Diomansil C18(4.6 mm×150 mm,5μm),流动相为甲醇-水-冰醋酸(77∶22∶1);检测波长为428 nm;流速为1.0 ml/min。血药浓度数据采用3P97药动学软件进行分析。结果:大黄酸血药浓度在1.0～15μg/ml范围内线性关系良好。大鼠给予大黄及调胃承气汤后,大黄酸血浆浓度-时间曲线均符合二房室模型,大黄及调胃承气汤的主要药动学参数AUC分别为12.06±1.34和4.19±0.48μg.h/ml、Cmax分别为7.53±1.13和2.58±0.21μg/ml,均存在显著性差异(P<0.05)。结论:调胃承气汤中大黄与甘草、芒硝配伍后,使大黄酸在大鼠体内的血药浓度降低。
Objective: To study the pharmacokinetics of rhein in rats after the rats were given rhubarb and Tiaocheng Chengqi Decoction. Methods: Rats were given rhubarb and Tiaocheng Chengqi Decoction, HPLC determination of rhein blood concentration. The column was Diomansil C18 (4.6 mm × 150 mm, 5 μm) and the mobile phase was methanol-water-glacial acetic acid (77:22:1). The detection wavelength was 428 nm and the flow rate was 1.0 ml / min. Plasma concentration data using 3P97 pharmacokinetic software for analysis. Results: Rhein plasma concentration in the range of 1.0 ~ 15μg / ml good linear relationship. After the rats were given rhubarb and Tiaocheng Chengqi Decoction, the plasma concentration-time curves of rhein both conformed to the two-compartment model. The main pharmacokinetic parameters AUC of rhubarb and Tiaocheng Chengqi Decoction were 12.06 ± 1.34 and 4.19 ± 0.48μg respectively. h / ml, Cmax were 7.53 ± 1.13 and 2.58 ± 0.21μg / ml respectively, there was significant difference (P <0.05). Conclusion: Tamay Cheng Chengqi Decoction Rhubarb and licorice, Glauber’s salt compatibility, the rhein in rat plasma concentration decreased.