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采用极谱仪技术研究了甘油和甘露醇对豚鼠耳蜗外淋巴氧分压(PO_2)的影响。方法是将豚鼠全麻后,行气管切开术,并施人工呼吸。经腹侧进路打开左侧听泡,充分暴露骨性耳蜗,在基底转鼓阶上方耳蜗骨壁开窗,约100μm,插入直径80μm氧敏感电极于鼓阶内,为了绝缘,听泡内注入琼脂,参考电极置于颈部肌肉。化学微量感受器通过数字显示获得氧流量,并通过多种波动描记器记录结果。经颈外静脉分别注射25%甘油(4.8ml/kg)和25%
The effect of glycerol and mannitol on the pressure of peroxynitric oxygen (PO_2) in cochlea of guinea pigs was studied by using polarography. Method is to guinea pig after general anesthesia, tracheotomy, and artificial respiration. The ventral access to open the left to listen to the bubble, fully exposed bony cochlea, the basal drum above the cochlear bone wall fenestration, about 100μm, inserted diameter 80μm oxygen sensitive electrode in the scala tympani, for insulation, Agar, reference electrode placed on the neck muscles. The chemical micropreceptors obtain the oxygen flow through the digital display and record the results through a variety of wavetiprays. The external jugular vein were injected with 25% glycerol (4.8ml / kg) and 25%